Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments

Gholamreza Abdi, Morteza Khush-hkhui, Akhtar Shekafandeh

Abstract


Abstract:A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100 mg·l-1), 4% sucrose and 8 g·l-1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse.


Keywords


Explant, In vitro, Glutamic Acid, MS medium, Tissue culture

Full Text:

PDF


DOI: http://dx.doi.org/10.13128/ahs-24090



This work is licensed under a Creative Commons Attribution 4.0 International License (CC-BY- 4.0)
 
Firenze University Press
Via Cittadella, 7 - 50144 Firenze
Tel. (0039) 055 2757700 Fax (0039) 055 2757712
E-mail: info@fupress.com