Seasonal variation of Prunus necrotic ringspot virus concentration in almond, peach, and plum cultivars

N. Salem, A. Mansour, A. Al-musa, A. Al-nsour


Levels of Prunus necrotic ringspot virus (PNRSV) infection in almond, peach, and plum cultivars over the
course of an entire year were determined by testing different plant parts of naturally infected trees, using the double
antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The data showed that spring was the best
time of year for PNRSV detection in flowers, active growing buds, and young leaves. PNRSV detection was less
reliable during the summer months. Young leaves of all cultivars were the most reliable source for distinguishing
between healthy and infected plants, while flowers and buds yielded high values in some cultivars but not in others.
Seasonal fluctuations in virus concentration did not follow the same pattern in all cultivars. It is therefore impossible
to distinguish between infected and healthy trees on the basis of one single sampling time for all cultivars.

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