Molecular Diagnostics and Variability of Longidorid Nematodes

Abstract

PCR-RFLP and sequencing approaches of ribosomal DNA are being used to study taxonomy, molecular
identification and phylogeny of plant parasitic nematodes. In this paper, we discuss on the usefulness of ITS PCRRFLP
analysis to differentiate among longidorid species. In addition, we examined how well ITS PCR-RFLP differentiated
between longidorid species, and how well sequencing of two different ribosomal regions, the ITS containing
region and D1-D2 domains of the 26S rDNA, were able to infer phylogenetic relationships among those same species.
These methods and their advantages in identifying longidorids and establishing their phylogenetic relationships are
examined and discussed.