Superoxide Dismutase as a Tool for the Mulacular Identification of Plant Parasitic Nematodes
Varying amounts of SOD activity have been found at all life stages of the most diffused plant parasitic nematodes.
SOD is important to aerobic metabolism and parasitism of nematodes in that it catalyzes the first step of the neutralization
of the highly toxic superoxide anion (O2
•-), which is largely produced in plant-nematode incompatible
reactions. SOD has also been shown to be a significant tool to diagnose root-knot, cyst-, and longidorid nematodes. A
high SOD polymorphism has been revealed by Native-Page on gradient polyacrylamide gels for Meloidogyne spp. and
by isoelectrofocusing for Globodera, Xiphinema and Longidorus spp. The sensitivity of such procedures has been
improved by using the PhastSystem (Amersham Biosciences, Piscata, NJ, USA), an automated equipment for electrophoresis.
An accurate discrimination of species of all the nematode genera tested has been achieved and an attempt
was made to group populations of the Xiphinema americanum-group and to detect Globodera rostochiensis
and G. pallida pathotypes.
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