Antagonistic Activity of Trichoderma ISolates against Sclerotium rolfsii : Screening of Efficient Isolates from Morocco Soils for Biological Control

N. Khattabi, B. Ezzahiri, L. Louali, A. Oihabi


Seventy Trichoderma spp. isolates collected from different regions of Morocco were tested for their capacity
to inhibit in vitro mycelial growth of Sclerotium rolfsii, and for their effect on the viability of S. rolfsii sclerotia in
the soil. The Trichoderma spp. isolates inhibited mycelial growth of S. rolfsii to various degrees, with 52% of isolates
expressing an average inhibition, varying between 45 and 55%. The effect on the viability of sclerotia in the soil also
varied between isolates of Trichoderma, with the majority (84%) having a slight effect. A group of twenty isolates
identified as Trichoderma harzianum when tested in sterilized soil, significantly reduced sclerotial viability though
not in natural soil. Four of these isolates (Nz, Kb2, Kb3 and Kf1) showed good antagonistic activity against S. rolfsii
and were also highly competitive in natural soil. These isolates would therefore be candidates for development in
biological control program.

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