Detection and Molecular Characterization of Grapevine Virus A in Jordan

G. Anfoka, W. Shahrour, M.K. Nakhla

Abstract


In a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was
detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found
that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995), a portion of the coat
protein gene of the virus was amplified by IC-RT-PCR and RT-PCR, using leaf extracts and RNA extracted from
infected grapevines respectively. After cloning and sequencing the coat protein gene of the Jordanian isolate of GVA
(GVA-Jo), the sequence of the amplified product was compared with sequences of other GVA isolates from different
countries.

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DOI: http://dx.doi.org/10.14601/Phytopathol_Mediterr-1764



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