Occurrence and Distribution of Citrus tristeza virus (CTV) in the Jordan Valley

G. Anfoka, M.K. Abhary, I. Fattash, M.K. Nakhla


In a survey conducted in 2002 and 2003, Citrus tristeza virus (CTV) was detected in the Jordan Valley.
The direct tissue blot immunoassay (DTBIA) indicated that 12.7 and 15.2% of samples tested in the central and
northern Jordan Valley respectively were infected with CTV. Similar results showed that all citrus species grown in
the Jordan Valley were susceptible to CTV. DAS-ELISA analysis of samples from a citrus orchard in the Dir Alla area
with severe CTV symptoms indicated that 49% of samples were infected with CTV. Using a CTV specific primer pair
(CTV1/CTV10), the coat protein gene of the virus was successfully amplified from leaf extracts obtained from CTVinfected
trees by IC-RT-PCR. After cloning and sequencing the coat protein gene, the sequence of the amplified
product was deposited in the GenBank.

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DOI: http://dx.doi.org/10.14601/Phytopathol_Mediterr-1779

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