Simple Techniques for Long-Term Storage of Plasmopara viticola

Abstract

Propagules of Plasmopara viticola or fragments of P. viticola infected host tissue were stored by three
techniques, freezing at –25°C, dehydration with CaCl2, and freeze-drying. Viability tests were performed periodically
until exhaustion of the testing sample. P. viticola samples that were frozen at -25°C remained viable for eight years,
whereas the CaCl2 dehydrated samples remained viable for eight and a half years. Freeze-drying was the most
successful long-term storage method, being able to preserve samples for almost 21 years.