Diseases caused by species of Botryosphaeriaceae lead to significant losses of grape yield. Species in this family produce foliar symptoms similar to, but distinguishable from esca, and the diseases they cause are generally named black dead arm (BDA). Botryosphaeriaceae species are ascomycetes frequently isolated from grapevine stocks showing decline or dieback symptoms. It is therefore useful to know what is the spore dissemination period of Botryosphaeriaceae in the vineyard. The objective of this study was to determine the peak periods of conidial release by some Diplodia spp. in the Botryosphaeriaceae in grapevines and to ascertain the climatic factors that influence inoculum availability and dispersal. Spore dispersal from Vitis vinifera was studied from 2005 to 2006 in a French vineyard. Spores of Diplodia seriata and D. mutila were trapped throughout the year. Spore release from D. seriata peaked during the vegetative growth period, while D. mutila released its spores later.